DPP4/CD26 Monoklonaler Antikörper

DPP4/CD26 Monoklonal Antikörper für WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen

Anwendung

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

2G6D11

Kat-Nr. : 68383-1-PBS

Synonyme

CD26, 2G6D11, ADABP, ADCP 2, ADCP2

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  WB analysis using 68383-1-Ig (same clone as 68383-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

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  WB analysis of HUVEC using 68383-1-Ig (same clone as 68383-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

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  WB analysis of HuH-7 using 68383-1-Ig (same clone as 68383-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 68383-1-Ig (same clone as 68383-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

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  WB analysis of pig kidney using 68383-1-Ig (same clone as 68383-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit kidney using 68383-1-Ig (same clone as 68383-1-PBS)

  rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50051-1

  Sandwich ELISA standard curve of MP50051-1, DPP4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68383-1-PBS. Detection antibody: 68383-2-PBS. Standard: Eg0157. Range: 0.313-20 ng/mL.

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  Sample test of MP50051-1

  Urine of eight individual healthy human donors was measured. The DPP4/CD26 concentration of detected samples was determined to be 33.23 ng/mL with a range of 9.07-105.84 ng/mL.

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  Sample test of MP50051-1

  Saliva of eight individual healthy human donors was measured. The DPP4/CD26 concentration of detected samples was determined to be 33.57 ng/mL with a range of 21.27-77.69 ng/mL.

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  Sample test of MP50051-1

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean DPP4/CD26 concentration was determined to be 0.17 ng/mL in unstimulated PBMC supernatant, 1.84 ng/mL in PHA stimulated PBMC supernatant.

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  Sample test of MP50051-1

  Serum of sixteen individual healthy human donors was measured. The DPP4/CD26 concentration of detected samples was determined to be 475.10 ng/mL with a range of 189.59-990.11 ng/mL.

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  IF Staining of HuH-7 using 68383-1-Ig (same clone as 68383-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using DPP4/CD26 antibody (68383-1-Ig, Clone: 2G6D11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68383-1-PBS bindet in WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test DPP4/CD26 und zeigt Reaktivität mit Hausschwein, human, Kaninchen

• Getestete Reaktivität: Hausschwein, human, Kaninchen
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DPP4/CD26 fusion protein Eg0157
• Vollständiger Name: dipeptidyl-peptidase 4
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC013329
• Gene symbol: DPP4
• Gene ID (NCBI): 1803
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).