DPYD Polyklonaler Antikörper

DPYD Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 27662-1-AP

Synonyme

DHP, DHPDHASE, Dihydrothymine dehydrogenase, Dihydrouracil dehydrogenase, DPD, DPYD

---

• 

  WB analysis of rat liver using 27662-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 27662-1-AP (DPYD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 27662-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 27662-1-AP (DPYD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 27662-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 27662-1-AP (DPYD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 27662-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27662-1-AP (DPYD antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27662-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27662-1-AP (DPYD antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

27662-1-AP bindet in WB, IHC, IF, ELISA DPYD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DPYD fusion protein Ag26599
• Vollständiger Name: dihydropyrimidine dehydrogenase
• Berechnetes Molekulargewicht: 1025 aa, 111 kDa
• Beobachtetes Molekulargewicht: 111 kDa
• GenBank-Zugangsnummer: BC008379
• Gene symbol: DPYD
• Gene ID (NCBI): 1806
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Pharmgenomics Pers Med; Exploration and Validation of Pancreatic Cancer Hub Genes Based on Weighted Gene Co-Expression Network Analysis and Immune Infiltration Score Analysis; Authors - Xiao-Xi Li
human	IHC	J Cell Mol Med; Pathway-based stratification of gliomas uncovers four subtypes with different TME characteristics and prognosis; Authors - Ruoyu Huang
human,mouse	IF	Theranostics; Single cell analyses reveal the PD-1 blockade response-related immune features in hepatocellular carcinoma; Authors - Yao Li
human	WB	J Clin Invest; CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes; Authors - Nunziata Maio
human	IHC	Nat Commun; Proteomic profiling of gliomas unveils immune and metabolism-driven subtypes with implications for anti-nucleotide metabolism therapy; Authors - Jinsen Zhang
human	WB	Clin Transl Oncol; Pyrimidine metabolism reshapes immune microenvironment and implies poor prognosis in glioma; Authors - Ruoyu Huang ; KD Validated