CRMP4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CRMP4 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13661-1-AP

Synonyme

CRMP 4, CRMP4, dihydropyrimidinase like 3, DPYSL3, DRP 3, DRP3, LCRMP, ULIP, ULIP 1, ULIP1, Unc 33 like phosphoprotein 1

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Galerie der Validierungsdaten

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  WB analysis using 13661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13661-1-AP (CRMP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 13661-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13661-1-AP (CRMP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 13661-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13661-1-AP (CRMP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13661-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13661-1-AP (CRMP4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13661-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13661-1-AP (CRMP4 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 13661-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13661-1-AP (CRMP4 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 13661-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13661-1-AP (CRMP4 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13661-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13661-1-AP (CRMP4 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13661-1-AP bindet in WB, IHC, IF, ELISA CRMP4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRMP4 fusion protein Ag4557
• Vollständiger Name: dihydropyrimidinase-like 3
• Berechnetes Molekulargewicht: 570 aa, 62 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC039006
• Gene symbol: DPYSL3
• Gene ID (NCBI): 1809
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DPYSL3(Dihydropyrimidinase-related protein 3) is also named as CRMP4, DRP3, ULIP, ULIP1 and belongs to the hydantoinase/dihydropyrimidinase subfamily.DPYSL3 is a developmentally regulated protein, strongly expressed in early embryonic post-mitotic neural cells, reaching a peak of expression around the first post-natal week. In the adult brain, DPYSL3 is present mainly in regions that retain neurogenesis.DPYSL3 protein is also found in synaptic sites at the adult neuromuscular junction where it may be involved in the maintenance of neuronal stability(PMID: 16135096).It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	Mol Neurobiol; Collapsin response mediator protein 5 (CRMP5) modulates susceptibility to chronic social defeat stress in mice.; Authors - Yu-Fen Lin
mouse	WB	Neuropharmacology; Collapsin response mediator protein 5 (CRMP5) causes social deficits and accelerates memory loss in an animal model of Alzheimer's disease.; Authors - Yung-Shuen Lin
human	IF,IHC	Cancer Biol Ther; CRMP4a suppresses cell motility by sequestering RhoA activity in prostate cancer cells.; Authors - Changlin Li ; KD Validated
mouse	WB	Int J Mol Sci; Elevated Hippocampal CRMP5 Mediates Chronic Stress-Induced Cognitive Deficits by Disrupting Synaptic Plasticity, Hindering AMPAR Trafficking, and Triggering Cytokine Release; Authors - Yu-Fen Lin