TNFRSF21/DR6 Monoklonaler Antikörper

TNFRSF21/DR6 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C2D10

Kat-Nr. : 66754-1-PBS

Synonyme

DR6, TNFRSF21, 3C2D10, CD358, Death receptor 6

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  WB analysis of K-562 using 66754-1-Ig (same clone as 66754-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66754-1-Ig (same clone as 66754-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66754-1-Ig (same clone as 66754-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 66754-1-Ig (same clone as 66754-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 66754-1-Ig (same clone as 66754-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66754-1-Ig (same clone as 66754-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DR6 antibody (66754-1-Ig, Clone: 3C2D10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66754-1-PBS bindet in WB, IF/ICC, Indirect ELISA TNFRSF21/DR6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TNFRSF21/DR6 fusion protein Ag14327
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 21
• Berechnetes Molekulargewicht: 655 aa, 72 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC021572
• Gene symbol: DR6
• Gene ID (NCBI): 27242
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNFRSF21 (also known as DR6 or CD358) is an orphan receptor of the tumor necrosis factor (TNF) receptor superfamily. It controls cell death and differentiation in a cell-autonomous manner in different cell types including neurons, oligodendrocytes, endothelial cells, and immune cells (PMID: 29459438). DR6 has been shown to activate NF-κB and JNK, and induce cell apoptosis (PMID: 9714541). DR6 interacts with TRADD, which is known to mediate signal transduction of TNF receptors. It has been reported that APP binds DR6 to trigger axon pruning and neuron death via distinct caspases, indicating that DR6 might be involved in Alzheimer's disease (PMID: 19225519).