DRD1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DRD1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 17934-1-AP

Synonyme

D(1A) dopamine receptor, DADR, Dopamine D1 receptor, dopamine receptor D1, DRD1, DRD1A

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 17934-1-AP

  37°C incubated mouse brain tissue were subjected to SDS PAGE followed by western blot with 17934-1-AP (DRD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17934-1-AP (DRD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17934-1-AP (DRD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17934-1-AP (DRD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 17934-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17934-1-AP (DRD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 17934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD1 antibody (17934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 17934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD1 antibody (17934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: bei 37°C inkubiertes Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Produktinformation

17934-1-AP bindet in WB, IHC, IF, ELISA DRD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DRD1 fusion protein Ag12366
• Vollständiger Name: dopamine receptor D1
• Berechnetes Molekulargewicht: 446 aa, 49 kDa
• Beobachtetes Molekulargewicht: 50-75 kDa
• GenBank-Zugangsnummer: BC074978
• Gene symbol: DRD1
• Gene ID (NCBI): 1812
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dopamine is a neurotransmitter that plays a crucial role in physical and mental health, such as cardiovascular, hormonal, renal and central nervous systems (PMID: 29220802). Five subtypes of mammalian dopamine receptors are grouped into two classes, the D1- and D2-like classes. The D1-like class includes D1 and D5 receptors whereas the D2-like class includes D2, D3, D4 subtypes (PMID: 9457173). Dopamine receptor D1 (DRD1) is the most abundant form of dopamine receptor in the central nervous system. DRD1 stimulates adenylate cyclase, modulates D2 receptor activity, regulates neuron growth and differentiation, and mediates several behavioral responses (PMID: 1977312). DRD1 has a calculated molecular weight of 49 kDa, larger apparent molecular weight of 60-80 kDa may be due to glycosylation (PMID: 1281547; 23821371).

Publikationen

Species	Application	Title
human	WB	Cell; PMID: 25594175; Authors - Yiqing Yan ; KD Validated
rat	WB	EMBO Mol Med; MicroRNA expression profile and functional analysis reveal that miR-382 is a critical novel gene of alcohol addiction.; Authors - Li Jingyuan J
human	IF	Elife; The organic cation transporter 2 regulates dopamine D1 receptor signaling at the Golgi apparatus.; Authors - Natasha M Puri
rat	WB	Int J Mol Sci; Effects of Maternal High-Fructose Diet on Long Non-Coding RNAs and Anxiety-like Behaviors in Offspring; Authors - Yuchen Zou
mouse	IF	CNS Neurosci Ther; Amphiregulin blockade decreases the levodopa-induced dyskinesia in a 6-hydroxydopamine Parkinson's disease mouse model; Authors - Piniel Alphayo Kambey
human	WB	Mol Neurobiol; Protective Effect of Curcumin by Modulating BDNF/DARPP32/CREB in Arsenic-Induced Alterations in Dopaminergic Signaling in Rat Corpus Striatum.; Authors - Pranay Srivastava

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