DRP1 (C-terminal) Rekombinanter Antikörper

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• KD/KO Validated

DRP1 (C-terminal) Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A19

Kat-Nr. : 81561-1-PBS

Synonyme

DRP1, 2A19, DLP1, DNM1L, DNM1L,DLP1

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  WB analysis using 81561-1-RR (same clone as 81561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 81561-1-RR (same clone as 81561-1-PBS)

  WB result of DRP1 (C-terminal) antibody (81561-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DRP1 (C-terminal) transfected HEK-293 cells. This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 81561-1-RR (same clone as 81561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

• 

  IP experiment of PC-12 using 81561-1-RR (same clone as 81561-1-PBS)

  IP result of anti-DRP1 (C-terminal) (IP:81561-1-RR, 4ug; Detection:81561-1-RR 1:5000) with PC-12 cells lysate 1560 ug. This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 81561-1-RR (same clone as 81561-1-PBS)

  IP result of anti-DRP1 (C-terminal) (IP:81561-1-RR, 4ug; Detection:81561-1-RR 1:5000) with rat brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 81561-1-RR (same clone as 81561-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 81561-1-RR (same clone as 81561-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 81561-1-RR (same clone as 81561-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using DRP1 (C-terminal) antibody (81561-1-RR, Clone: 2A19 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 81561-1-RR (same clone as 81561-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DRP1 (C-terminal) (81561-1-RR, Clone:2A19) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81561-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81561-1-RR against Human DRP1 (C-terminal) were performed. The affinity constant is 0.825 nM.

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Geprüfte Anwendungen

Produktinformation

81561-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA DRP1 (C-terminal) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DRP1 (C-terminal) fusion protein Ag3644
• Vollständiger Name: dynamin 1-like
• Berechnetes Molekulargewicht: 736 aa, 82 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC024590
• Gene symbol: DRP1
• Gene ID (NCBI): 10059
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.