Desmocollin 2 Polyklonaler Antikörper

Desmocollin 2 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Rezensionen (3)

Kat-Nr. : 13876-1-AP

Synonyme

DSC2, Desmocollin-3, Desmocollin-2, Desmocollin 3, CDHF2

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  WB analysis using 13876-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 10x lens).

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  IHC staining of human oesophagus using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of mouse heart using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 13876-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Desmocollin 2 antibody (13876-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 13876-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Desmocollin 2 antibody (13876-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophagusgewebe, humanes Hautkrebsgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

13876-1-AP bindet in WB, IHC, IF-P, ELISA Desmocollin 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Desmocollin 2 fusion protein Ag4956
• Vollständiger Name: desmocollin 2
• Berechnetes Molekulargewicht: 100 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC063291
• Gene symbol: Desmocollin 2
• Gene ID (NCBI): 1824
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Desmocollin 2 (DSC2) is a member of the desmocollin subfamily of the cadherin superfamily. The desmocollins (DSC1-3), along with the desmogleins (DSG1-4), are found primarily in epithelial and cardiac muscle where they constitute the adhesive proteins of the intercellular desmosome junctions and are required for cell adhesion and desmosome formation. Mutations in the DSC2 gene are associated with arrhythmogenic right ventricular dysplasia-11.

Publikationen

Species	Application	Title
human	IF	Dev Cell; Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model.; Authors - Sally Dabelsteen
mouse	WB	Sci Total Environ; Environmental cadmium impairs blood-testis barrier via activating HRI-responsive mitochondrial stress in mice.; Authors - Guo-Xiang Zhou
human	IF	Br J Cancer; Mapping of truncated O-glycans in cancers of epithelial and non-epithelial origin.; Authors - Troels Boldt Rømer
human	WB	Bioconjug Chem; Transforms of Cell Surface Glycoproteins Charge Influences Tumor Cell Metastasis via Atypically Inhibiting Epithelial-Mesenchymal Transition Including Matrix Metalloproteinases and Cell Junctions; Authors - Mingzhe Wang
human	IHC	Mol Ther Oncolytics; Exosomal miR-205-5p enhances angiogenesis and nasopharyngeal carcinoma metastasis by targeting desmocollin-2.; Authors - Wenjuan Yang
human	WB,IHC,IF	BMC Cancer; Desmoglein 2 and desmocollin 2 depletions promote malignancy through distinct mechanisms in triple-negative and luminal breast cancer; Authors - Ji-Yuan Han ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chi (Verified Customer) (08-18-2025)

The antibody was used to detect Desmocollin in human iPSC-derived cardiomyocytes by IF. It worked just fine, but it gave us significant background noise.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: human iPSC-derived cardiomyocytes

Desmocollin 2 Antibody Immunofluorescence validation (1:200 dilution) in human iPSC-derived cardiomyocytes (Cat no:13876-1-AP)

FH

Kiara (Verified Customer) (07-28-2025)

This primary antibody did not pass internal validation for IF and it did indeed not give us a reliable signal for IF of methanol fixed iPSC-cardiomyocytes.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: iPSC-derived cardiomyocytes

Desmocollin 2 Antibody Immunofluorescence validation (1:400 dilution) in iPSC-derived cardiomyocytes (Cat no:13876-1-AP)

FH

Jane (Verified Customer) (02-03-2022)

One specific band was found around 120kDa, however, the signal is weak so need to use lower dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Heart tissue