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DSE Monoklonaler Antikörper

DSE Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C9F7

Kat-Nr. : 60041-1-PBS

Synonyme

dermatan sulfate epimerase, DS epimerase, DSE, DSEPI, SART 2, SART2

Filter:
  • Western Blot (WB) analysis of COLO 320 cells using DSE Monoclonal antibody (60041-1-Ig)

    WB analysis of COLO 320 using 60041-1-Ig (same clone as 60041-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60041-1-Ig (DSE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DSE Monoclonal antibody (60041-1-Ig)

    IHC staining of human liver cancer using 60041-1-Ig (same clone as 60041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60041-1-Ig (DSE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DSE Monoclonal antibody (60041-1-Ig)

    IHC staining of human liver cancer using 60041-1-Ig (same clone as 60041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60041-1-Ig (DSE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DSE Monoclonal antibody (60041-1-Ig)

    IF Staining of HeLa using 60041-1-Ig (same clone as 60041-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSE antibody (60041-1-Ig, Clone: 2C9F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60041-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

60041-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DSE und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgM
Klonalität Monoklonal
Typ Antikörper
Immunogen DSE fusion protein Ag0695
Vollständiger Name dermatan sulfate epimerase
Berechnetes Molekulargewicht 958 aa, 110 kDa
Beobachtetes Molekulargewicht 100 kDa
GenBank-ZugangsnummerBC039245
Gene symbol DSE
Gene ID (NCBI) 29940
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Thiophiler Affinitätschromatograph
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DSE, also named as SART2 and DSEPI, is an enzyme that converts D-glucuronic acid to L-iduronic acid residues in dermatan sulphate biosynthesis. It is also identified to be a tumour-associated antigen. DSE is recognized by cytotoxic T cells (CTLs) and its enhanced expression in many cancers has been reported. DSE is a potential candidate for a tumour antigen with immunogenicity.