DSG1 Polyklonaler Antikörper

DSG1 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 24587-1-AP

Synonyme

Cadherin family member 4, CDHF4, desmoglein 1, Desmoglein-1, Desmosomal glycoprotein 1

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  IHC staining of human skin cancer using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of human skin cancer using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of mouse skin using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 24587-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DSG1 antibody (24587-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushautgewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 11 publications below

Produktinformation

24587-1-AP bindet in WB, IHC, IF/ICC, ELISA DSG1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DSG1 fusion protein Ag20184
• Vollständiger Name: desmoglein 1
• Berechnetes Molekulargewicht: 1049 aa, 114 kDa
• GenBank-Zugangsnummer: BC153001
• Gene symbol: DSG1
• Gene ID (NCBI): 1828
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG1 is a single-pass transmembrane glycoprotein highly expressed in the epidermis and localized primarily within the suprabasal epithelial layers (PMID: 16286477; 24220297). DSG1 mediates intercellular adhesion and is crucial in maintaining epidermal integrity and barrier function (PMID: 23974871). It is also involved in epithelial cell differentiation (PMID: 23524961). Mutations in the DSG1 gene can cause the autosomal dominant disorder to striate palmoplantar keratoderma and a syndrome featuring severe dermatitis, multiple allergies, and metabolic wasting (SAM syndrome) (PMID: 29315490; 23974871).

Publikationen

Species	Application	Title
mouse	WB,IHC	J Nanobiotechnology; ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis; Authors - Xuan Lai
human	IF	EMBO Rep; O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.; Authors - Ieva Bagdonaite
human	IF	Stem Cell Res Ther; Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.; Authors - Ling Leng
	IF	J Cell Sci; Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.; Authors - Valentina Iadevaia
human	WB,IF	In Vitro Cell Dev Biol Anim; S100A11 is involved in the progression of colorectal cancer through the desmosome-catenin-TCF signaling pathway; Authors - Jin Zhou
mouse	WB,IF	Microscopy (Oxf); Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.; Authors - Shoko Ishikawa