DSG1 Polyklonaler Antikörper

DSG1 Polyklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24587-1-PBS

Synonyme

Cadherin family member 4, CDHF4, desmoglein 1, Desmoglein-1, Desmosomal glycoprotein 1

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  IHC staining of human skin cancer using 24587-1-AP (same clone as 24587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 24587-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 24587-1-AP (same clone as 24587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 24587-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 24587-1-AP (same clone as 24587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24587-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 24587-1-AP (same clone as 24587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24587-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 24587-1-AP (same clone as 24587-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DSG1 antibody (24587-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24587-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24587-1-PBS bindet in IHC, IF/ICC, Indirect ELISA DSG1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DSG1 fusion protein Ag20184
• Vollständiger Name: desmoglein 1
• Berechnetes Molekulargewicht: 1049 aa, 114 kDa
• GenBank-Zugangsnummer: BC153001
• Gene symbol: DSG1
• Gene ID (NCBI): 1828
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG1 is a single-pass transmembrane glycoprotein highly expressed in the epidermis and localized primarily within the suprabasal epithelial layers (PMID: 16286477; 24220297). DSG1 mediates intercellular adhesion and is crucial in maintaining epidermal integrity and barrier function (PMID: 23974871). It is also involved in epithelial cell differentiation (PMID: 23524961). Mutations in the DSG1 gene can cause the autosomal dominant disorder to striate palmoplantar keratoderma and a syndrome featuring severe dermatitis, multiple allergies, and metabolic wasting (SAM syndrome) (PMID: 29315490; 23974871).