DSG3 Polyklonaler Antikörper

DSG3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29942-1-PBS

Synonyme

PVA, DSG 3, Desmoglein-3, Desmoglein 3, CDHF6

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  WB analysis using 29942-1-AP (same clone as 29942-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29942-1-AP (DSG3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse tongue using 29942-1-AP (same clone as 29942-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 29942-1-AP (DSG3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse tongue using 29942-1-AP (same clone as 29942-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 29942-1-AP (DSG3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung squamous cell carcinoma using 29942-1-AP (same clone as 29942-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 29942-1-AP (DSG3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

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  IF Staining of HaCaT using 29942-1-AP (same clone as 29942-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using DSG3 antibody (29942-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

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  IF Staining of HaCaT using 29942-1-AP (same clone as 29942-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using DSG3 antibody (29942-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 29942-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29942-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DSG3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DSG3 fusion protein Ag30880
• Vollständiger Name: desmoglein 3 (pemphigus vulgaris antigen)
• Berechnetes Molekulargewicht: 108 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: NM_001944
• Gene symbol: DSG3
• Gene ID (NCBI): 1830
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DSG3, Desmoglein-3, is the component of intercellular desmosome junctions. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. DSG3 is expressed in the basal lower layers of in the skin epidermis (PMID: 30528827). It acts as a prognostic marker of Esophageal Squamous cell carcinoma (PMID:24568510).