DSP Monoklonaler Antikörper

DSP Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B10D4

Kat-Nr. : 68364-1-PBS

Synonyme

desmoplakin, DP, DPI, DPII, DSP

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  WB analysis using 68364-1-Ig (same clone as 68364-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68364-1-Ig (DSP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68364-1-Ig (same clone as 68364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68364-1-Ig (DSP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68364-1-Ig (same clone as 68364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68364-1-Ig (DSP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68364-1-Ig (same clone as 68364-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DSP antibody (68364-1-Ig, Clone: 1B10D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68364-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68364-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DSP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DSP fusion protein Ag30499
• Vollständiger Name: desmoplakin
• Berechnetes Molekulargewicht: 2871 aa, 332 kDa
• Beobachtetes Molekulargewicht: 240-330 kDa
• GenBank-Zugangsnummer: BC140802
• Gene symbol: DSP
• Gene ID (NCBI): 1832
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)