DSPP Polyklonaler Antikörper

DSPP Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 55396-1-AP

Synonyme

Dentin phosphophoryn, dentin sialophosphoprotein, DFNA39, DGI1, DMP3, DPP, DPP Dentin sialoprotein, DSP, DSPP, DTDP2

---

• 

  WB analysis using 55396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55396-1-AP (DSPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 55396-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSPP antibody (55396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 55396-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSPP antibody (55396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, LNCaP-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

55396-1-AP bindet in WB, IF/ICC, ELISA DSPP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: dentin sialophosphoprotein
• Berechnetes Molekulargewicht: 131 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: NM_014208
• Gene symbol: DSPP
• Gene ID (NCBI): 1834
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DSPP (dentin sialophosphoprotein) is a precursor protein belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. DSPP is secreted by odontoblasts and then is leaved into 3 fragments: N-terminal dentin sialoprotein (DSP), intermediate dentin glycoprotein (DGP) and C-terminal dentin phosphoprotein (DPP) (PMID:22201843). DSP is heavily glycosylated but DPP is not. DSP and DPP are principle proteins of the dentin extracellular matrix of the tooth, with DSP having a role in dentinogenesis and DPP binding calcium, facilitating initial mineralization of dentin matrix collagen and regulating the size and shape of the crystals. The molecular mass of full-length is around 130kDa(uniprot), the cleavage product of DSPP with a molecular mass of around 90 to 100 kDa (PMID: 21103065).

Publikationen

Species	Application	Title
human	WB	Int J Nanomedicine; Multifunctional Lithium-Doped Mesoporous Nanoparticles for Effective Dentin Regeneration in vivo; Authors - Zitian Liang
human	WB	Arch Oral Biol; NOTUM plays a bidirectionally modulatory role in the odontoblastic differentiation of human stem cells from the apical papilla through the WNT/β-catenin signaling pathway; Authors - Qingxuan Zhao
mouse	WB	Eur J Pharmacol; Vdr mediates Wnt signaling pathway to regulate odontoblasts differentiation during dentin apposition; Authors - Yinlin Wu
human	WB	Int Endod J; Effects of phase-transited lysozyme on adhesion, migration and odontogenic differentiation of human dental pulp cells: An in vitro study; Authors - Jiange Zhao