DSTYK Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DSTYK Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 20102-1-AP

Synonyme

RIPK5, Receptor-interacting serine/threonine-protein kinase 5, KIAA0472, HDCMD38P, EC:2.7.12.1

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  WB analysis using 20102-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20102-1-AP (DSTYK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20102-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DSTYK antibody (20102-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

20102-1-AP bindet in WB, IHC, IF/ICC, ELISA DSTYK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DSTYK fusion protein Ag13678
• Vollständiger Name: dual serine/threonine and tyrosine protein kinase
• Berechnetes Molekulargewicht: 66 kDa, 105 kDa
• Beobachtetes Molekulargewicht: 66 kDa, 100~105 kDa
• GenBank-Zugangsnummer: BC060870
• Gene symbol: DSTYK
• Gene ID (NCBI): 25778
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DSTYK (Dual serine-threonine and tyrosine protein kinase gene), also known as CAKUT1, DustyPK, SPG23 has been classically considered as RIPK5, encodes a protein product consisting of 850-931 amino acids, which has a striking membrane-associated distribution in mesenchymal-derived cells of all major organs including brain, heart, kidney, lung, colon, and muscle (PMID: 34853310). DSTYK is strongly expressed in the cytoplasm of most malignant cells and that the high expression of DSTYK in human colon cancer specimens is associated with tumor progression and/or poor prognosis.

Publikationen

Species	Application	Title
monkey	IF	Nat Commun; Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.; Authors - Xianding Sun
human,mouse	WB,IHC	Cell Death Dis; Loss of DSTYK activates Wnt/β-catenin signaling and glycolysis in lung adenocarcinoma; Authors - Chenxi Zhong ; KD Validated
human		EMBO Rep; DSTYK phosphorylates STING at late endosomes to promote STING signaling; Authors - Hao Dong