DTL Polyklonaler Antikörper

DTL Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 12896-1-AP

Synonyme

CDT2, CDW1, DCAF2, DDB1- and CUL4-associated factor 2, Denticleless protein homolog

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  WB analysis of HeLa using 12896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12896-1-AP (DTL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12896-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12896-1-AP (DTL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12896-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12896-1-AP (DTL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12896-1-AP

  IP result of anti-DTL (IP:12896-1-AP, 3ug; Detection:12896-1-AP 1:500) with mouse testis tissue lysate 8000ug.

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  IF Staining of HeLa using 12896-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12896-1-AP (DTL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, L02-Zellen, MCF-7-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

12896-1-AP bindet in WB, IF/ICC, IP, ELISA DTL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DTL fusion protein Ag3589
• Vollständiger Name: denticleless homolog (Drosophila)
• Berechnetes Molekulargewicht: 730 aa, 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC033540
• Gene symbol: DTL
• Gene ID (NCBI): 51514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDT2, also named as L2DTL or RAMP, is one of the substrate receptors of the Cullin Ring Ubiquitin Ligase 4 that targets for ubiquitin mediated degradation a number of substrates, such as CDT1, p21 and CHK1, involved in the regulation of cell cycle and survival. CDT2 overexpression was reported in breast (PMID: 18542055), gastric (PMID: 19672268) and ovarian carcinomas (PMID: 23995842) and rhabdomyosarcomas (PMID: 19235922) and associated with the aggressiveness of hepatocellular carcinomas (PMID: 17106265).

Publikationen

Species	Application	Title
mouse	IF	Sci Adv; Profiling and functional characterization of maternal mRNA translation during mouse maternal-to-zygotic transition.; Authors - Chunxia Zhang
		Dev Dyn; Denticleless E3 ubiquitin protein ligase (DTL) maintains the proliferation and differentiation of epidermis and hair follicles during skin development; Authors - Yanhui Lin
human	WB	Proteomics; Solvent-induced proteome profiling for proteomic quantitation and target discovery of small molecular drugs; Authors - Chengli Yu