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DTL Rekombinanter Antikörper

DTL Rekombinant Antikörper für IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230144C9

Kat-Nr. : 83107-1-PBS

Synonyme

Denticleless protein homolog, DDB1- and CUL4-associated factor 2, DCAF2, CDW1, CDT2

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DTL Recombinant antibody (83107-1-RR)

    IF Staining of HeLa using 83107-1-RR (same clone as 83107-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DTL antibody (83107-1-RR, Clone: 230144C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83107-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using DTL Recombinant antibody (83107-1-RR)

    FC experiment of HeLa using 83107-1-RR (same clone as 83107-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DTL Recombinant antibody (83107-1-RR, Clone:230144C9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83107-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

83107-1-PBS bindet in IF/ICC, FC (Intra), Indirect ELISA DTL und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen DTL fusion protein Ag33391
Vollständiger Name denticleless homolog (Drosophila)
Berechnetes Molekulargewicht 730 aa, 79 kDa
GenBank-ZugangsnummerBC033540
Gene symbol DTL
Gene ID (NCBI) 51514
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDT2, also named as L2DTL or RAMP, is one of the substrate receptors of the Cullin Ring Ubiquitin Ligase 4 that targets for ubiquitin mediated degradation a number of substrates, such as CDT1, p21 and CHK1, involved in the regulation of cell cycle and survival. CDT2 overexpression was reported in breast (PMID: 18542055), gastric (PMID: 19672268) and ovarian carcinomas (PMID: 23995842) and rhabdomyosarcomas (PMID: 19235922) and associated with the aggressiveness of hepatocellular carcinomas (PMID: 17106265).