DTX3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DTX3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 25304-1-AP

Synonyme

deltex homolog 3 (Drosophila), Deltex3, DTX3, Protein deltex 3, RING finger protein 154, RNF154

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Galerie der Validierungsdaten

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  WB analysis of hTERT-RPE1 using 25304-1-AP

  Western blot analysis in wildtype and DTX3 knockouted human hTERT-RPE1 cells with 25304-1-AP (DTX3 antibody). The data provided by an anonymous customer.

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  WB analysis using 25304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 25304-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 25304-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25304-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 25304-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25304-1-AP (DTX3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 25304-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25304-1-AP (DTX3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 25304-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25304-1-AP (DTX3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 25304-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25304-1-AP (DTX3 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, hTERT-RPE1-Zellen, Maus-Eierstockgewebe, Maushirngewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below

Produktinformation

25304-1-AP bindet in WB, IHC, ELISA DTX3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DTX3 fusion protein Ag18111
• Vollständiger Name: deltex homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 347 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC114498
• Gene symbol: DTX3
• Gene ID (NCBI): 196403
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DTX3, also named as Deltex3 or RING finger protein 154, is 347 amino acid protein, which contains one RING-type zinc finger and belongs to the Deltex family. DTX3 is a regulator of Notch signaling, which is involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations.

Publikationen

Species	Application	Title
human	WB	J Cancer; Deltex3 inhibits Epithelial Mesenchymal Transition in Papillary Thyroid Carcinoma via promoting ubiquitination of XRCC5 to regulate the AKT signal pathway.; Authors - Lidong Wang ; KD Validated
human	WB	Mol Biol Rep; Deltex E3 ubiquitin ligase 3 inhibits colorectal cancer cell growth and regulates cell cycle progression via upregulating E2F transcription factor 1.; Authors - Hongli Xu ; KO Validated