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DTX4 Polyklonaler Antikörper

DTX4 Polyklonal Antikörper für WB, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25222-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of mouse kidney tissue using DTX4 Polyclonal antibody (25222-1-AP)

    WB analysis of mouse kidney using 25222-1-AP (same clone as 25222-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25222-1-AP (DTX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using DTX4 Polyclonal antibody (25222-1-AP)

    WB analysis of mouse kidney using 25222-1-AP (same clone as 25222-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25222-1-AP (DTX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25222-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse embryo tissue using DTX4 Polyclonal antibody (25222-1-AP)

    IF Staining of mouse embryo using 25222-1-AP (same clone as 25222-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 25222-1-AP (DTX4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25222-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DTX4 Polyclonal antibody (25222-1-AP)

    IF Staining of HEK-293 using 25222-1-AP (same clone as 25222-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 25222-1-AP (DTX4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25222-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25222-1-PBS bindet in WB, IF/ICC, IF-P, Indirect ELISA DTX4 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DTX4 fusion protein Ag18415
Vollständiger Name deltex homolog 4 (Drosophila)
Berechnetes Molekulargewicht 619 aa, 67 kDa
Beobachtetes Molekulargewicht 67-70 kDa
GenBank-ZugangsnummerBC119011
Gene symbol DTX4
Gene ID (NCBI) 23220
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DTX4, also named as KIAA0937, RNF155, is a 619 amino acid protein, which belongs to the Deltex family. DTX4 as a regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. DTX4 functions as a ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4.