DTYMK Polyklonaler Antikörper

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• KD/KO Validated

DTYMK Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15360-1-AP

Synonyme

CDC8, dTMP kinase, DTYMK, Thymidylate kinase, TMPK, TYMK

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15360-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 15360-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15360-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15360-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15360-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15360-1-AP

  IP Result of anti-DTYMK (IP:15360-1-AP, 3ug; Detection:15360-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human colon cancer using 15360-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15360-1-AP (DTYMK antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 15360-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15360-1-AP (DTYMK antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 15360-1-AP

  Immunofluorescent analysis of MCF-7 cells, using DTYMK antibody 15360-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 15360-1-AP

  Immunofluorescent analysis of HepG2 cells, using DTYMK antibody 15360-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of MCF-7 using 15360-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug DTYMK antibody (15360-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Herzgewebe, humanes Hirngewebe, Maushirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Produktinformation

15360-1-AP bindet in WB, IP, IHC, IF, FC, ELISA DTYMK und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DTYMK fusion protein Ag7606
• Vollständiger Name: deoxythymidylate kinase (thymidylate kinase)
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC001827
• Gene symbol: DTYMK
• Gene ID (NCBI): 1841
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DTYMK(Thymidylate kinase) is also named as CDC8,TMPK and TYMK.It catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis.This enzyme is important in cellular DNA synthesis because the synthesis of dTTP either via the de novo pathway or through the exogenous supply of thymidine requires the activity of this enzyme(PMID:18469).

Publikationen

Species	Application	Title
human	WB, IHC	Cancer Res; Targeting p300/CBP attenuates hepatocellular carcinoma progression through epigenetic regulation of metabolism.; Authors - Ling-Yan Cai ; KD Validated
human	WB	Cells; Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line.; Authors - Madhu Kollareddy
human	WB	Front Mol Biosci; Deoxythymidylate Kinase as a Promising Marker for Predicting Prognosis and Immune Cell Infiltration of Pan-cancer.; Authors - Tianfeng Lan
human	WB	Proteomes; Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.; Authors - Henrick Horita
human	WB,IHC	Cell Death Dis; Inhibition of DTYMK significantly restrains the growth of HCC and increases sensitivity to oxaliplatin.; Authors - Fengze Sun ; KD Validated