DTYMK Rekombinanter Antikörper

DTYMK Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251441C1

Kat-Nr. : 86543-3-RR

Synonyme

CDC8, dTMP kinase, EC:2.7.4.9, TMPK, TYMK

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  WB analysis using 86543-3-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 86543-3-RR (DTYMK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 86543-3-RR

  rat brain tissue were subjected to SDS PAGE followed by western blot with 86543-3-RR (DTYMK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86543-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86543-3-RR against Human DTYMK were performed. The affinity constant is 0.565 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, K-562-Zellen, MOLT-4-Zellen, Raji-Zellen, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86543-3-RR bindet in WB, ELISA DTYMK und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DTYMK fusion protein Ag7606
• Vollständiger Name: deoxythymidylate kinase (thymidylate kinase)
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC001827
• Gene symbol: DTYMK
• Gene ID (NCBI): 1841
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DTYMK(Thymidylate kinase) is also named as CDC8,TMPK and TYMK.It catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis.This enzyme is important in cellular DNA synthesis because the synthesis of dTTP either via the de novo pathway or through the exogenous supply of thymidine requires the activity of this enzyme(PMID:18469).