DUOX1 Monoklonaler Antikörper

DUOX1 Monoklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

3B8D6

Rezensionen (1)

Kat-Nr. : 67226-1-Ig

Synonyme

3B8D6, DUOX, DUOX 1, EC:1.11.1.-, Large NOX 1

---

• 

  WB analysis of human testis using 67226-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67226-1-Ig (DUOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67226-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67226-1-Ig (DUOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 67226-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67226-1-Ig (DUOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse stomach using 67226-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using DUOX1 antibody (67226-1-Ig, Clone: 3B8D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of rat lung using 67226-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using DUOX1 antibody (67226-1-Ig, Clone: 3B8D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IF-P: Maus-Magengewebe, Rattenlungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

67226-1-Ig bindet in WB, IF-P, ELISA DUOX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DUOX1 fusion protein Ag18154
• Vollständiger Name: dual oxidase 1
• Berechnetes Molekulargewicht: 1551 aa, 177 kDa
• Beobachtetes Molekulargewicht: 190-200 kDa, 177 kDa, 138 kDa
• GenBank-Zugangsnummer: BC114938
• Gene symbol: DUOX1
• Gene ID (NCBI): 53905
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DUOX1 (dual oxidase 1) is a calciumactivated transmembrane protein member of the NOX family of NADPH oxidases principally located on the apical surface of airway and thyroid epithelia (PMID: 28982074, 15677770, 10806195). DUOX1 is a strong generator of hydrogen peroxide (H2O2), via intra-molecular dismutation of superoxide (PMID: 25586178). Epithelial DUOX1 appears to participate in additional host defense actions, such as mucociliary clearance, and may contribute to inflammatory responses and epithelial repair processes during exogenous stress (PMID: 19386603). DUOX1 has two isofroms with the calculated molecular mass of 177 and 138 kDa, and apparent molecular mass of 180-200 kDa due to the glycosylation (PMID: 17643428, 19144650).

Publikationen

Species	Application	Title
human	IF	Cancers (Basel); Patient-Derived Papillary Thyroid Cancer Organoids for Radioactive Iodine Refractory Screening.; Authors - Luc H J Sondorp
human	WB	BMC Mol Cell Biol; Upregulated dual oxidase 1-induced oxidative stress and caspase-1-dependent pyroptosis reflect the etiologies of heart failure; Authors - Yan Song Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Edma (Verified Customer) (03-03-2025)

The antibody is not that specific either in EB or in the IF images

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 (WB), 1:200 (IF)
• Cell Tissue Type: mouse lung homogenate (WB), mouse lungs (IF)