DUSP11 Polyklonaler Antikörper

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• KD/KO Validated

DUSP11 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 10204-2-AP

Synonyme

RNA/RNP complex-1-interacting phosphatase, PIR1, EC:3.1.3.-, DUSP 11, Dual specificity protein phosphatase 11

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  WB analysis using 10204-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10204-2-AP (DUSP11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10204-2-AP

  IP result of anti-DUSP11 (IP:10204-2-AP, 4ug; Detection:10204-2-AP 1:4000) with K-562 cells lysate 1760 ug.

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  IF Staining of A431 using 10204-2-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DUSP11 antibody (10204-2-AP) at dilution of 1:1400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:700-1:2800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below

Produktinformation

10204-2-AP bindet in WB, IF/ICC, IP, ELISA DUSP11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DUSP11 fusion protein Ag0276
• Vollständiger Name: dual specificity phosphatase 11 (RNA/RNP complex 1-interacting)
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC000346
• Gene symbol: DUSP11
• Gene ID (NCBI): 8446
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DUSP11, also known as PIR1, is a unique member of atypical DUSPs which could bind directly to RNA and possess RNA 5'-triphosphatase and diphosphatase activities. DUSP11 converts the 5' triphosphate of microRNA precursors to a 5' monophosphate, and regulates cellular noncoding RNAs levels. In addition to a catalysis towards RNA, more evidence showed that DUSP11 could also dephosphorylate proteins.

Publikationen

Species	Application	Title
human	WB	Ann Rheum Dis; Novel autoantibodies identified in ACPA-negative rheumatoid arthritis.; Authors - Ketian Li
human	WB	Nat Commun; RIG-I like receptor sensing of host RNAs facilitates the cell-intrinsic immune response to KSHV infection.; Authors - Yang Zhao
human	WB	Proc Natl Acad Sci U S A; RNA triphosphatase DUSP11 enables exonuclease XRN-mediated restriction of hepatitis C virus.; Authors - Rodney P Kincaid ; KO Validated
human	WB	Genes Dev; DUSP11 activity on triphosphorylated transcripts promotes Argonaute association with noncanonical viral microRNAs and regulates steady-state levels of cellular noncoding RNAs.; Authors - James M Burke
human	WB	Virol Sin; HBV precore G1896A mutation promotes growth of hepatocellular carcinoma cells by activating ERK/MAPK pathway; Authors - Baoxin Zhao ; KD Validated
human	WB	RNA Biol; DUSP11 - an RNA phosphatase that regulates host and viral non-coding RNAs in mammalian cells.; Authors - James M Burke

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