DUSP16 Polyklonaler Antikörper

DUSP16 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 14237-1-AP

Synonyme

DUSP16, KIAA1700, MAP kinase phosphatase 7, MKP 7, MKP7

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  WB analysis of PC-12 using 14237-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of COLO 320 using 14237-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of mouse brain using 14237-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 14237-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human colon using 14237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 14237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 14237-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 14237-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, COLO 320-Zellen, HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

14237-1-AP bindet in WB, IHC, ELISA DUSP16 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DUSP16 fusion protein Ag5479
• Vollständiger Name: dual specificity phosphatase 16
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC042101
• Gene symbol: DUSP16
• Gene ID (NCBI): 80824
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DUSP16, also known as MKP-7, is a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. DUSP16 dephosphorylates JUN kinases (JNKs) specifically and thereby regulates T-cell differentiation, which can be responsible for the pathogenesis of Burkitt's lymphoma and cerebral ischemia. DUSP16 was also found to induce the expression of VCAM-1 through the suppression of JNK signaling.

Publikationen

Species	Application	Title
mouse	WB	Cell Death Discov; NR4A1 enhances MKP7 expression to diminish JNK activation induced by ROS or ER-stress in pancreatic β cells for surviving.; Authors - Ze-Qing Pu
human	WB	Int Immunopharmacol; Berberine derivative DCZ0358 induce oxidative damage by ROS-mediated JNK signaling in DLBCL cells; Authors - Qilin Feng
human,mouse	IHC	J Mol Cell Cardiol; Deficiency of smooth muscle cell ILF3 alleviates intimal hyperplasia via HMGB1 mRNA degradation-mediated regulation of the STAT3/DUSP16 axis; Authors - Ya-Min Hou
human	WB	Int J Hyperthermia; Deregulated JNK signaling enhances apoptosis during hyperthermia; Authors - Atsushi Enomoto
human	WB	Cell Stress Chaperones; JNK Signaling Dominance in Hyperthermia; Authors - Atsushi Enomoto