DUSP2 Polyklonaler Antikörper

DUSP2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 27327-1-AP

Synonyme

dual specificity phosphatase 2, DUSP2, PAC 1, PAC1

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Galerie der Validierungsdaten

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  WB analysis of A549 using 27327-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27327-1-AP (DUSP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27327-1-AP (DUSP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27327-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27327-1-AP (DUSP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• FC: See 1 publications below

Produktinformation

27327-1-AP bindet in WB, FC, ELISA DUSP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DUSP2 fusion protein Ag25380
• Vollständiger Name: dual specificity phosphatase 2
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC007771
• Gene symbol: DUSP2
• Gene ID (NCBI): 1844
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DUSP2 belongs to the sub-family of nuclear specific DUSPs that includes DUSP1, 4 and 5. DUSP2 is highly expressed in immune cells, in particular, of patients with inflammatory arthritis, such as in rheumatoid arthritis (RA), and Dusp2 knockout mice present a significant reduction in inflammatory responses. It has been demonstrated that DUSP2 is a downstream target of the tumor suppressor p53 that is required for the cellular response to oxidative stress. DUSP2 is also involved in transcription factor E2F1-mediated apoptotic signaling. Like other members of the DUSP family, DUSP2 interacts with MAPKs; However, the target protein for its phosphatase activity is controversial.

Publikationen

Species	Application	Title
human	WB	Hum Cell; Exosomal hsa_circ_0017252 attenuates the development of gastric cancer via inhibiting macrophage M2 polarization.; Authors - Jin Song
mouse	WB	Basic Clin Pharmacol Toxicol; Apatinib combined with Keytruda treatment induces apoptosis of gastric carcinoma cells through CES4/miR-616-5p/DUSP2 axis.; Authors - Fengli Zhang
human	FC	Blood Coagul Fibrinolysis; Effects of Momordica charantia exosomes on platelet activation, adhesion, and aggregation.; Authors - Jianchao Zhang
mouse	WB	Bioact Mater; Large extracellular vesicles secreted by human iPSC-derived MSCs ameliorate tendinopathy via regulating macrophage heterogeneity; Authors - Teng Ye
mouse	WB	Cell Rep; GABA regulates IL-1β production in macrophages; Authors - Jian Fu ; KD Validated