DUSP6 Polyklonaler Antikörper

DUSP6 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26071-1-PBS

Synonyme

MKP3, MKP 3, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, EC:3.1.3.16

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  WB analysis using 26071-1-AP (same clone as 26071-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26071-1-AP (DUSP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-12 using 26071-1-AP (same clone as 26071-1-PBS)

  IP result of anti-DUSP6 (IP:26071-1-AP, 4ug; Detection:26071-1-AP 1:1000) with PC-12 cells lysate 1040 ug. This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 26071-1-AP (same clone as 26071-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 26071-1-AP (DUSP6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 26071-1-AP (same clone as 26071-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DUSP6 antibody (26071-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 26071-1-AP (same clone as 26071-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using DUSP6 antibody (26071-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 26071-1-AP (same clone as 26071-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DUSP6 Polyclonal antibody (26071-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 26071-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

26071-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA DUSP6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DUSP6 fusion protein Ag23474
• Vollständiger Name: dual specificity phosphatase 6
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC003562
• Gene symbol: DUSP6
• Gene ID (NCBI): 1848
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).