DUSP6 Rekombinanter Antikörper

DUSP6 Rekombinant Antikörper für FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240303A9

Kat-Nr. : 83355-3-PBS

Synonyme

240303A9, dual specificity phosphatase 6, EC:3.1.3.16, MAP kinase phosphatase 3, Mitogen-activated protein kinase phosphatase 3

---

• 

  FC experiment of PC-12 using 83355-3-RR (same clone as 83355-3-PBS)

  1x10^6 PC-12 cells were intracellularly stained with 0.25 ug Dusp6 Recombinant Antibody (83355-3-RR, Clone:240303A9) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83355-3-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83355-3-RR (same clone as 83355-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Dusp6 Recombinant Antibody (83355-3-RR, Clone:240303A9) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83355-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00391-2

  Cytometric bead array standard curve of MP00391-2, DUSP6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83355-3-PBS. Detection antibody: 83355-4-PBS. Standard: Ag0705. Range: 5-80ng/mL.

• 

  Affinity and Kinetic Characterization of 83355-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83355-3-PBS against Human DUSP6 was performed. The affinity constant is 41.7 pM.

• 

  Affinity and Kinetic Characterization of 83355-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83355-3-RR against Human DUSP6 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

83355-3-PBS bindet in FC (Intra), Cytometric bead array, Indirect ELISA DUSP6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DUSP6 fusion protein Ag0705
• Vollständiger Name: dual specificity phosphatase 6
• Berechnetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC003562
• Gene symbol: DUSP6
• Gene ID (NCBI): 1848
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).