DUSP7/PYST2 Polyklonaler Antikörper

DUSP7/PYST2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 26910-1-AP

Synonyme

dual specificity phosphatase 7, DUSP7, MKP X, MKPX, PYST2

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Galerie der Validierungsdaten

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  WB analysis of rat heart using 26910-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 26910-1-AP (DUSP7/PYST2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 26910-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26910-1-AP (DUSP7/PYST2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 26910-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26910-1-AP (DUSP7/PYST2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 26910-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26910-1-AP (DUSP7/PYST2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26910-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26910-1-AP (DUSP7/PYST2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 26910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26910-1-AP (DUSP7/PYST2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 26910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26910-1-AP (DUSP7/PYST2 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenherzgewebe, Mausherzgewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

26910-1-AP bindet in WB, IHC, ELISA DUSP7/PYST2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DUSP7/PYST2 fusion protein Ag25488
• Vollständiger Name: dual specificity phosphatase 7
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC104880
• Gene symbol: DUSP7
• Gene ID (NCBI): 1849
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human dual-specificity phosphatase 7 (DUSP7/PYST2) is a member of a structurally homologous subfamily of MAP kinase phosphatases. DUSP7 is overexpressed in myeloid leukemia and other malignancies. It is known to bind and dephosphorylate ErkII, and as it, along with the other members of the DUSP family expresses high selectively for MAP kinases. It has been suggested that it functions as a method for selectively activating/deactivating different members of that family.

Publikationen

Species	Application	Title
mouse	WB,IHC	Clin Transl Med; Loss of SETD2 aggravates colorectal cancer progression caused by SMAD4 deletion through the RAS/ERK signalling pathway; Authors - Chunxiao Ma
human	WB	Mol Cancer; Linc-RoR promotes MAPK/ERK signaling and confers estrogen-independent growth of breast cancer.; Authors - Wan-Xin Peng
human	WB	Br J Pharmacol; L-Hcy-induced cathepsin V mediates the vascular endothelial inflammation in hyperhomocysteinemia.; Authors - Yi-Ping Leng
mouse	WB	Addict Biol; Dual-specificity phosphatase 15 (DUSP15) in the nucleus accumbens is a novel negative regulator of morphine-associated contextual memory.; Authors - Xiaomeng Qiao
mouse	WB	Genes Cells; Downregulation of DUSP6, a negative regulator of oncogenic ERK signaling, by ACA-28 induces apoptosis in NIH/3T3 cells overexpressing HER2/ErbB2.; Authors - Yuki Kanda