DVL1 Polyklonaler Antikörper

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• KD/KO Validated

DVL1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 27384-1-AP

Synonyme

Dishevelled 1, DSH homolog 1, DVL, DVL1

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Galerie der Validierungsdaten

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  WB analysis using 27384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 27384-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 27384-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 27384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DVL1 antibody (27384-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HEK-293-Zellen, Maus-Cerebellum-Gewebe, MDA-MB-231-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Produktinformation

27384-1-AP bindet in WB, IHC, IF, ELISA DVL1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DVL1 fusion protein Ag26055
• Vollständiger Name: dishevelled, dsh homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 73 kDa
• GenBank-Zugangsnummer: BC050454
• Gene symbol: DVL1
• Gene ID (NCBI): 1855
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway.

Publikationen

Species	Application	Title
human	IHC	Theranostics; Transcription factor AP-4 promotes tumorigenic capability and activates the Wnt/β-catenin pathway in hepatocellular carcinoma.; Authors - Junwei Song
mouse	WB	Brain Res Bull; Excess folic acid supplementation before and during pregnancy and lactation activates β-catenin in the brain of male mouse offspring.; Authors - Qian Wu
rat	WB	Neural Plast; Lycopene-Loaded Microemulsion Regulates Neurogenesis in Rats with Aβ-Induced Alzheimer's Disease Rats Based on the Wnt/β-catenin Pathway.; Authors - Wen-Jing Ning
mouse	WB	Biol Trace Elem Res; Selenium Deficiency Leads to Changes in Renal Fibrosis Marker Proteins and Wnt/β-Catenin Signaling Pathway Components.; Authors - Tingting Lin
rat	WB	Neurosci Lett; Dishevelled-1 regulated apoptosis through NF-κB in cerebral ischemia/reperfusion injury in rats.; Authors - Xiaogang Zhang ; KD Validated
human	IHC	J Obstet Gynaecol; cDNA expression library screening revealed novel functional genes involved in clear cell carcinogenesis of the ovary in vitro.; Authors - Yasushi Yamada