DVL1 Polyklonaler Antikörper

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• KD/KO Validated

DVL1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27384-1-PBS

Synonyme

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  WB analysis using 27384-1-AP (same clone as 27384-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27384-1-AP (same clone as 27384-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 27384-1-AP (same clone as 27384-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 27384-1-AP (same clone as 27384-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 27384-1-AP (same clone as 27384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27384-1-AP (same clone as 27384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 27384-1-AP (same clone as 27384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 27384-1-AP (same clone as 27384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 27384-1-AP (same clone as 27384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 27384-1-AP (same clone as 27384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 27384-1-AP (same clone as 27384-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DVL1 antibody (27384-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27384-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DVL1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DVL1 fusion protein Ag26055
• Vollständiger Name: dishevelled, dsh homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 73 kDa
• GenBank-Zugangsnummer: BC050454
• Gene symbol: DVL1
• Gene ID (NCBI): 1855
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway.