DVL1 Monoklonaler Antikörper

DVL1 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A2A5

Kat-Nr. : 67672-1-Ig

Synonyme

Dishevelled 1, DSH homolog 1, DVL, DVL1

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Galerie der Validierungsdaten

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  WB analysis using 67672-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67672-1-Ig (DVL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 67672-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 67672-1-Ig (DVL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 67672-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67672-1-Ig (DVL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat colon using 67672-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 67672-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 67672-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 67672-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, MDA-MB-231-Zellen, NCCIT-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: Ratten-Kolongewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67672-1-Ig bindet in WB, IHC, ELISA DVL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DVL1 fusion protein Ag26083
• Vollständiger Name: dishevelled, dsh homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC050454
• Gene symbol: DVL1
• Gene ID (NCBI): 1855
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway. There are two isoforms of DVL1: 75 kDa and 73 kDa.