DVL3 Polyklonaler Antikörper

DVL3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13444-1-PBS

Synonyme

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  WB analysis using 13444-1-AP (same clone as 13444-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13444-1-AP (DVL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 13444-1-AP (same clone as 13444-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13444-1-AP (DVL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Raji using 13444-1-AP (same clone as 13444-1-PBS)

  IP result of anti-DVL3 (IP:13444-1-AP, 4ug; Detection:13444-1-AP 1:500) with Raji cells lysate 1440ug. This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 13444-1-AP (same clone as 13444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13444-1-AP (same clone as 13444-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DVL3 antibody (13444-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13444-1-AP (same clone as 13444-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13444-1-AP (DVL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13444-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13444-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DVL3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DVL3 fusion protein Ag4272
• Vollständiger Name: dishevelled, dsh homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 716 aa, 78 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC032459
• Gene symbol: DVL3
• Gene ID (NCBI): 1857
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.