DYKDDDDK tag Rekombinanter Antikörper

DYKDDDDK tag Rekombinant Antikörper für IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein

Anwendung

IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4K14

Kat-Nr. : 80010-1-PBS

Synonyme

Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK

---

• 

  IP experiment of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  IP result of anti-DYKDDDDK tag (IP:80010-1-RR, 4ug; Detection:80010-1-RR 1:3000) with Transfected HEK-293 cells lysate 400 ug. This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 (eGFP-DYKDDDDK transfected) cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), eGFP (Green), DYKDDDDK (Red), DAPI (Blue). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFP (green). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 80010-1-RR (DDDDK tag antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 80010-1-RR (DYKDDDDK tag antibody), at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope) (80010-1-RR, Clone:4K14) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 80010-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80010-1-RR against DYKDDDDK tag were performed. The affinity constant is 83.7 pM.

---

Geprüfte Anwendungen

Produktinformation

80010-1-PBS bindet in IF/ICC, IP, Indirect ELISA DYKDDDDK tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DYKDDDDK tag fusion protein Ag2329
• Vollständiger Name: DYKDDDDK tag
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.