DYKDDDDK tag Rekombinanter Antikörper

DYKDDDDK tag Rekombinant Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

240568A11

Rezensionen (1)

Kat-Nr. : 80801-2-RR

Synonyme

3Flag, Flag, DDDDKtag, DDDDK tag, DDDDK

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  WB analysis of Transfected HEK-293T using 80801-2-RR

  TDP-43-flag Transfected HEK-293T cells were subjected to SDS PAGE followed by western blot with 80801-2-RR (Ag2329 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Transfected HEK-293 using 80801-2-RR

  IP result of anti-DYKDDDDK tag (IP:80801-2-RR, 4ug; Detection:80801-2-RR 1:3000) with Transfected HEK-293 cells lysate 400 ug.

• 

  IF Staining of Transfected HEK-293T using 80801-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293T cells using DYKDDDDK tag antibody (80801-2-RR, Clone: 240568A11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of Transfected HEK-293T using 80801-2-RR

  1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.25 ug DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope) (80801-2-RR, Clone:240568A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 80801-2-RR

  Biolayer interferometry (BLl) kinetic assays of 80801-2-RR against DYKDDDDK tag were performed. The affinity constant is 15.3 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: TDP-43-flag Transfected HEK-293T cells
• Erfolgreiche IP: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: Transfected HEK-293T cells
• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

80801-2-RR bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA DYKDDDDK tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DYKDDDDK tag fusion protein Ag2329
• Vollständiger Name: DYKDDDDK tag
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit recombinant antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Publikationen

Species	Application	Title
	WB,IF,IP	Virus Res; ZFP36 Facilitates Senecavirus A (SVA) Replication by Inhibiting the Production of Type I Interferon; Authors - Mengge Yin
mouse	IHC,IF	FASEB J; The SLE-associated TREX1-P212fs mutation disrupts ER association leading to type I interferonopathy; Authors - Shan Xu
mouse	WB,IP	Redox Biol; Gut flora-derived succinate exacerbates Allergic Airway Inflammation by promoting protein succinylation; Authors - Chao Wang
	WB	Cell Rep; High-glucose-associated YTHDC1 lactylation reduces the sensitivity of bladder cancer to enfortumab vedotin therapy; Authors - Zhuo Xing

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jai (Verified Customer) (06-25-2025)

I use this antibody very frequently for WBs as my constructs are usually FLAG tagged. Very reliable and almost no background labelling. Very good price as well compared to competitors

• Applications: Western Blot