DYKDDDDK tag Rekombinanter Antikörper
DYKDDDDK tag Rekombinant Antikörper für IF/ICC
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
rekombinanten Protein und mehr (1)
Anwendung
IF/ICC
Konjugation
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4K14
Kat-Nr. : CL488-80010
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in IF/ICC | Transfizierte HEK-293-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
IF | See 2 publications below |
Produktinformation
CL488-80010 bindet in IF/ICC DYKDDDDK tag und zeigt Reaktivität mit rekombinanten Protein
Getestete Reaktivität | rekombinanten Protein |
In Publikationen genannte Reaktivität | human |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Rekombinant |
Typ | Antikörper |
Immunogen | DYKDDDDK tag fusion protein Ag2329 |
Vollständiger Name | DYKDDDDK tag |
Gene symbol | |
Gene ID (NCBI) | |
Konjugation | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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IF protocol for CL Plus 488 DYKDDDDK tag antibody CL488-80010 | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther Chimeric antigen receptor with novel intracellular modules improves antitumor performance of T cells | ||
Theranostics Designing multifunctional recombinant vaccines: an engineering strategy based on innovative epitope prediction-guided splicing |