DYKDDDDK tag Rekombinanter Antikörper

DYKDDDDK tag Rekombinant Antikörper für WB

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

4K14

Kat-Nr. : CL750-80010

Synonyme

DDDDK, Flag tag, Flag, DYKDDDDK, DDDDK tag

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  WB analysis using CL750-80010

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-80010 (DYKDDDDK tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  FC experiment of Transfected HEK-293 using CL750-80010

  1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope) (CL750-80010, Clone:4K14)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche Detektion in FC (Intra): Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL750-80010 bindet in WB DYKDDDDK tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DYKDDDDK tag fusion protein Ag2329
• Vollständiger Name: DYKDDDDK tag
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.