DYNC1H1 Polyklonaler Antikörper

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• KD/KO Validated

DYNC1H1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12345-1-PBS

Synonyme

DYNC1H 1

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• 

  WB analysis of HeLa using 12345-1-AP (same clone as 12345-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1  antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12345-1-AP (same clone as 12345-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12345-1-AP (same clone as 12345-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1  antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12345-1-AP (same clone as 12345-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1  antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12345-1-AP (same clone as 12345-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1  antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 12345-1-AP (same clone as 12345-1-PBS)

  IP result of anti-DYNC1H1 (IP:12345-1-AP, 4ug; Detection:12345-1-AP 1:5000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12345-1-AP (same clone as 12345-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1  antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12345-1-AP (same clone as 12345-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1  antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 12345-1-AP (same clone as 12345-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 12345-1-AP (same clone as 12345-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12345-1-AP (same clone as 12345-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 12345-1-AP (DYNC1H1  antibody at dilution of 1:50. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12345-1-AP (same clone as 12345-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 12345-1-AP (DYNC1H1  antibody at dilution of 1:50. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12345-1-AP (same clone as 12345-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 12345-1-AP (DYNC1H1  antibody at dilution of 1:50. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 12345-1-AP (same clone as 12345-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DYNC1H1 antibody (12345-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 12345-1-AP (same clone as 12345-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using DYNC1H1 antibody 12345-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12345-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DYNC1H1 und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DYNC1H1 fusion protein Ag2999
• Vollständiger Name: dynein, cytoplasmic 1, heavy chain 1
• Berechnetes Molekulargewicht: 4646 aa, 532 kDa
• Beobachtetes Molekulargewicht: 532 kDa
• GenBank-Zugangsnummer: BC021297
• Gene symbol: DYNC1H1
• Gene ID (NCBI): 1778
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dyneins are a group of microtubule-activated ATPases that serve to convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients..