DYNC1I2 Polyklonaler Antikörper

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• KD/KO Validated

DYNC1I2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12219-1-PBS

Synonyme

IC2, DNCIC2, DNCI2, DH IC-2, DH IC 2

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  WB analysis of Jurkat using 12219-1-AP (same clone as 12219-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12219-1-AP (same clone as 12219-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 12219-1-AP (same clone as 12219-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 12219-1-AP (same clone as 12219-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 12219-1-AP (same clone as 12219-1-PBS)

  IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 12219-1-AP (same clone as 12219-1-PBS)

  IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with HeLa cells lysate 2080 ug. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 12219-1-AP (same clone as 12219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 12219-1-AP (same clone as 12219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 12219-1-AP (same clone as 12219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 12219-1-AP (same clone as 12219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 12219-1-AP (same clone as 12219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 12219-1-AP (same clone as 12219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A375 using 12219-1-AP (same clone as 12219-1-PBS)

  Immunofluorescent analysis of A375 cells using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12219-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DYNC1I2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DYNC1I2 fusion protein Ag2873
• Vollständiger Name: dynein, cytoplasmic 1, intermediate chain 2
• Berechnetes Molekulargewicht: 612 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68-72 kDa
• GenBank-Zugangsnummer: BC015038
• Gene symbol: DYNC1I2
• Gene ID (NCBI): 1781
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.