DYNLT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DYNLT1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 11954-1-AP

Synonyme

TCTEL1, T-complex testis-specific protein 1 homolog, Protein CW-1, Protein CW 1, Dynein light chain Tctex-type 1

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  WB analysis of human brain using 11954-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11954-1-AP (DYNLT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11954-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11954-1-AP (DYNLT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Sp2/0 using 11954-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 11954-1-AP (DYNLT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 11954-1-AP

  IP result of anti-DYNLT1 (IP:11954-1-AP, 4ug; Detection:11954-1-AP 1:300) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human pancreas using 11954-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11954-1-AP (DYNLT1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 11954-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11954-1-AP (DYNLT1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse testis using 11954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DYNLT1 antibody (11954-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 11954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DYNLT1 antibody (11954-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 11954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DYNLT1 antibody (11954-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, humanes Herzgewebe, Sp2/0-Zellen
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:600
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

11954-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA DYNLT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DYNLT1 fusion protein Ag2557
• Vollständiger Name: dynein, light chain, Tctex-type 1
• Berechnetes Molekulargewicht: 113 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC029412
• Gene symbol: DYNLT1
• Gene ID (NCBI): 6993
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DYNLT (or Tctex-1) was originally described as a light chain component of the dynein motor complex. Tctex-1 also has several dynein-independent functions, including roles in G protein signaling activation and neuronal growth. Tctex-1 is selectively enriched in proliferating neural progenitors of both embryonic and adult brains. Genetic knockdown of Tctex-1 in radial precursors promoted neurogenesis, indicating its implication in regulation of cortical neurogenesis. (Pubmed: 19448628)

Publikationen

Species	Application	Title
	IF	Nanomicro Lett; Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling; Authors - Junji Ren
mouse	WB,IHC,IF	Nat Neurosci; Lfc and Tctex-1 regulate the genesis of neurons from cortical precursor cells.; Authors - Gauthier-Fisher Andrée A ; KD Validated
mouse	WB	Cell Res; Dlic1 deficiency impairs ciliogenesis of photoreceptors by destabilizing dynein.; Authors - Kong Shanshan S
human	WB	J Cell Biol; Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.; Authors - Hironori Inaba ; KD Validated
human	IHC	Cancers (Basel); Dynein Light Chain Protein Tctex1: A Novel Prognostic Marker and Molecular Mediator in Glioblastoma.; Authors - Claudia Alexandra Dumitru
	WB	Biochem Pharmacol; Cannabinoid receptor-2 expression modulates Gβ1γ2 protein interaction with the activator of G protein signalling 2/dynein light chain protein Tctex-1.; Authors - Marina Nagler