DYRK2 Polyklonaler Antikörper

DYRK2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21979-1-AP

Synonyme

Dual specificity tyrosine-phosphorylation-regulated kinase 2, DYRK 2, EC:2.7.12.1

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  WB analysis using 21979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21979-1-AP (DYRK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 21979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using DYRK2 antibody (21979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, K-562-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IF/ICC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21979-1-AP bindet in WB, IF/ICC, ELISA DYRK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DYRK2 fusion protein Ag13480
• Vollständiger Name: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 67~70 kDa
• GenBank-Zugangsnummer: BC006375
• Gene symbol: DYRK2
• Gene ID (NCBI): 8445
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DYRK (Dual-specificity tyrosine phosphorylation-regulated kinase 2) is the only member of the Dual-specificity tYrosine phosphorylation-Regulated Kinase (DYRK) family that functions as a kinase activity-independent scaffold for an E3 ubiquitin ligase complex (PMID: 33376136). DYRK2 is involved in cell survival, development, differentiation, gene transcription, proteasomal regulation, and microtubule formation. DYRK2 is a key regulator of cell cycle and apoptosis in response to DNA damage via phosphorylation of p53. DYRK2 has two isoforms and is mostly localized in the cytoplasm; however, it accumulates in the nucleus under genotoxic stress conditions (PMID: 31505048, 37886981).