DZIP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DZIP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 13779-1-AP

Synonyme

DZIP, DAZ-interacting zinc finger protein 1, DAZ-interacting protein 1/2, DAZ interacting protein 1/2, DAZ interacting protein 1

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  WB analysis of HEK-293 using 13779-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13779-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 13779-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13779-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13779-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13779-1-AP

  IP result of anti-DZIP1 (IP:13779-1-AP, 4ug; Detection:13779-1-AP 1:300) with HEK-293 cells lysate 880ug.

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  IHC staining of human oesophagus cancer using 13779-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13779-1-AP (DZIP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13779-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13779-1-AP (DZIP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 13779-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13779-1-AP (DZIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Hirngewebe, humanes Hodengewebe, Maus-Skelettmuskelgewebe, PC-3-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 3 publications below

Produktinformation

13779-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DZIP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DZIP1 fusion protein Ag4772
• Vollständiger Name: DAZ interacting protein 1
• Berechnetes Molekulargewicht: 867 aa, 98 kDa
• Beobachtetes Molekulargewicht: 100-113 kDa
• GenBank-Zugangsnummer: BC041804
• Gene symbol: DZIP1
• Gene ID (NCBI): 22873
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DZIP1, also named as DZIP, DZIP2 and KIAA0996, belongs to the DZIP C2H2-type zinc-finger protein family. DZIP1 has been reported to be associated with centrosomes and ciliogenesis in cells (PMID: 32051257). DZIP1 may participate in spermatogenesis via its interaction with DAZ1, and play a role in primary formation (PMID: 15081113, 19852954). DZIP1 has 3 isoforms with the molecular mass of 31, 97 and 99 kDa. It may be a potential therapeutic target of oral squamous carcinoma (PMID: 31450126).

Publikationen

Species	Application	Title
mouse	IF	Nat Genet; Mutations in DZIP1L, which encodes a ciliary-transition-zone protein, cause autosomal recessive polycystic kidney disease.; Authors - Hao Lu
human	IF	Dev Biol; A function for the Joubert syndrome protein Arl13b in ciliary membrane extension and ciliary length regulation.; Authors - Hao Lu
mouse,human	WB,IF	Dev Dyn; DZIP1 regulates mammalian cardiac valve development through a Cby1-β-catenin mechanism.; Authors - Lilong Guo ; KO Validated