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DeltaN p63 Rekombinanter Antikörper

DeltaN p63 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251209D11

Kat-Nr. : 86154-11-PBS

Synonyme

p63, TP63, CUSP, TP 63, Transformation-related protein 63

Filter:
  • Western Blot (WB) analysis of various lysates using DeltaN p63 Recombinant monoclonal antibody (86154-11-RR)

    WB analysis using 86154-11-RR (same clone as 86154-11-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86154-11-RR (TP63 DeltaN(P40) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86154-11-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DeltaN p63 Recombinant monoclonal antibody (86154-11-RR)

    WB analysis using 86154-11-RR (same clone as 86154-11-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86154-11-RR (TP63 DeltaN(P40) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86154-11-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DeltaN p63 Recombinant monoclonal antibody (86154-11-RR)

    IF Staining of A431 using 86154-11-RR (same clone as 86154-11-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TP63 DeltaN(P40) antibody (86154-11-RR, Clone: 251209D11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86154-11-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86154-11-PBS bindet in WB, IF/ICC, Indirect ELISA DeltaN p63 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name tumor protein p63
Berechnetes Molekulargewicht66 kDa
Beobachtetes Molekulargewicht68-75 kDa
GenBank-ZugangsnummerNM_001114980
Gene symbol TP63
Gene ID (NCBI) 8626
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DeltaN p63 (ΔNp63) is a major protein isoform encoded by the TP63 gene. The TP63 gene is a member of the p53 gene family, which also includes p53 and p73. The key defining feature of DeltaN p63 (ΔNp63) is the absence of the full N-terminal transactivation domain. However, it retains the central DNA-binding domain (DBD) and the C-terminal oligomerization domain (OD). The α-isoform also has a sterile alpha motif (SAM) domain and a transcriptional inhibitory domain (TID). DeltaN p63 isoforms can act as dominant-negative inhibitors of p53 and TAp63 by competing for DNA binding sites or forming non-functional dimers. DeltaN p63 is highly expressed in basal layers of epithelial tissues and is essential for the maintenance and proliferation of epithelial stem cells. It plays a pivotal role in epidermal development, stratification, and regeneration.