DeltaN p63 Rekombinanter Antikörper

DeltaN p63 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251209D11

Kat-Nr. : 86154-11-RR

Synonyme

p63, TP63, CUSP, TP 63, Transformation-related protein 63

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  WB analysis using 86154-11-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-11-RR (TP63 DeltaN(P40) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86154-11-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-11-RR (TP63 DeltaN(P40) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 86154-11-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TP63 DeltaN(P40) antibody (86154-11-RR, Clone: 251209D11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A431-Zellen, HaCaT-Zellen, Maushautgewebe, PC-12-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86154-11-RR bindet in WB, IF/ICC, ELISA DeltaN p63 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tumor protein p63
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 68-75 kDa
• GenBank-Zugangsnummer: NM_001114980
• Gene symbol: TP63
• Gene ID (NCBI): 8626
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DeltaN p63 (ΔNp63) is a major protein isoform encoded by the TP63 gene. The TP63 gene is a member of the p53 gene family, which also includes p53 and p73. The key defining feature of DeltaN p63 (ΔNp63) is the absence of the full N-terminal transactivation domain. However, it retains the central DNA-binding domain (DBD) and the C-terminal oligomerization domain (OD). The α-isoform also has a sterile alpha motif (SAM) domain and a transcriptional inhibitory domain (TID). DeltaN p63 isoforms can act as dominant-negative inhibitors of p53 and TAp63 by competing for DNA binding sites or forming non-functional dimers. DeltaN p63 is highly expressed in basal layers of epithelial tissues and is essential for the maintenance and proliferation of epithelial stem cells. It plays a pivotal role in epidermal development, stratification, and regeneration.