Desmin Monoklonaler Antikörper

Desmin Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B1A11

Kat-Nr. : 67793-1-PBS

Synonyme

2B1A11, DES

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  WB analysis using 67793-1-Ig (same clone as 67793-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 67793-1-Ig (same clone as 67793-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 67793-1-Ig (same clone as 67793-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67793-1-Ig (same clone as 67793-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67793-1-Ig (same clone as 67793-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 67793-1-Ig (same clone as 67793-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 67793-1-Ig (same clone as 67793-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

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  IF Staining of human appendicitis using 67793-1-Ig (same clone as 67793-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 67793-1-Ig (same clone as 67793-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67793-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Desmin und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Desmin fusion protein Ag9675
• Vollständiger Name: desmin
• Berechnetes Molekulargewicht: 470 aa, 54 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC032116
• Gene symbol: Desmin
• Gene ID (NCBI): 1674
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.