Desmin Rekombinanter Antikörper

Desmin Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D21

Kat-Nr. : 82748-1-PBS

Synonyme

1D21, DES

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  WB analysis using 82748-1-RR (same clone as 82748-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82748-1-RR (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82748-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 82748-1-RR (same clone as 82748-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82748-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 82748-1-RR (same clone as 82748-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82748-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 82748-1-RR (same clone as 82748-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82748-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 82748-1-RR (same clone as 82748-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82748-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 82748-1-RR (same clone as 82748-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Desmin antibody (82748-1-RR, Clone: 1D21 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82748-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat heart using 82748-1-RR (same clone as 82748-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using Desmin antibody (82748-1-RR, Clone: 1D21 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82748-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82748-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82748-1-RR against Human Desmin were performed. The affinity constant is 1.32 nM.

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Geprüfte Anwendungen

Produktinformation

82748-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Desmin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Desmin fusion protein Ag9742
• Vollständiger Name: desmin
• Berechnetes Molekulargewicht: 470 aa, 54 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC032116
• Gene symbol: Desmin
• Gene ID (NCBI): 1674
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.