DFNA5/GSDME Rekombinanter Antikörper

DFNA5/GSDME Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251415B1

Kat-Nr. : 82955-2-PBS

Synonyme

Dfna5, Dfna5h, EG14210, Fin15, Gasdermin-E

---

• 

  WB analysis using 82955-2-RR (same clone as 82955-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82955-2-RR (Dfna5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82955-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP02686-1

  The mean DFNA5/GSDME concentration was determined to be 85.78 ng/mL in J744A.1 cell extract based on a 1.3 mg/mL extract load, 16.86 ng/mL in mouse brain tissue extract based on a 7.0 mg/mL extract load and 10.69 ng/mL in rat brain tissue extract based on a 8.2 mg/mL extract load.

• 

  Sandwich ELISA standard curve of MP02686-1

  Sandwich ELISA standard curve of MP02686-1, Mouse DFNA5/GSDME Recombinant Matched Antibody Pair - PBS only. 82955-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33622. 82955-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

• 

  Affinity and Kinetic Characterization of 82955-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82955-2-RR against Mouse DFNA5/GSDME were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

82955-2-PBS bindet in WB, Sandwich ELISA, Indirect ELISA DFNA5/GSDME und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DFNA5/GSDME fusion protein Ag33622
• Vollständiger Name: deafness, autosomal dominant 5 (human)
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC132303
• Gene symbol: DFNA5/GSDME
• Gene ID (NCBI): 54722
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.