Di-Methyl-Histone H3 (Lys56) Rekombinanter Antikörper

Di-Methyl-Histone H3 (Lys56) Rekombinant Antikörper für WB, Dot Blot, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Dot Blot, ELISA

Konjugation

Unkonjugiert

CloneNo.

243027A1

Kat-Nr. : 82828-12-RR

Synonyme

Di Methyl Histone H3, Di-Methyl Histone H3 (Lys56), Di-Methyl Histone H3 Lys56, Di-Methyl Histone-H3 Lys56, Di-Methyl-Histone H3

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  WB analysis using 82828-12-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82828-12-RR (HIST1H3A(H3K56me2) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Dot Blot experiment using 82828-12-RR

  Dot blot analysis was used to confirm the specificity of 82828-12-RR (Di-Methyl-Histone H3 (Lys56) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in Dot Blot: peptide

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• DOT BLOT: DOT BLOT : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82828-12-RR bindet in WB, Dot Blot, ELISA Di-Methyl-Histone H3 (Lys56) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Beobachtetes Molekulargewicht: 15-18 kDa
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816).