DjC8 Polyklonaler Antikörper

DjC8 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32709-1-AP

Synonyme

DnaJ homolog subfamily C member 8, DNAJC8, HSPC315, HSPC331, SPF31

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  WB analysis using 32709-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32709-1-AP (DNAJC8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, Caco-2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32709-1-AP bindet in WB, ELISA DjC8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DjC8 fusion protein Ag38520
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily C, member 8
• Berechnetes Molekulargewicht: 30kDa，253aa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: NM_014280.2
• Gene symbol: DNAJC8
• Gene ID (NCBI): 22826
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DjC8 (C8, a member of DNAJ heat shock protein family), also known as DNAJC8, which is a highly conserved protein, belonging to heat shock protein 40(HSP40) family. The protein is expressed in many cell types, including central nervous system macrophages, cerebellar inhibitory neurons, choroid plexus epithelial cells of ventricles, oligodendrocyte precursor cells and so on. In normal tissues, the expression level of DjC8 is relatively low, but it is high in some tissues such as testis, skeletal muscle and spleen. In hepatocellular carcinoma, the high expression of DjC8 is related to poor prognosis. Knocking down DjC8 can inhibit the proliferation, migration and invasion of hepatocellular carcinoma cells, and reduce the IC50 value of sorafenib, indicating that it may be a potential therapeutic target and prognostic marker for hepatocellular carcinoma. In addition, DjC8 is regulated by TIG1 in cervical cancer and participates in glycolysis. DjC8 contains a conserved J domain, which can interact with HSP70 and activate its ATPase activity, thus participating in the folding, unfolding, transport and degradation of protein. The molecular weight of DjC8 is 30 kDa.