Drebrin Polyklonaler Antikörper

Drebrin Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 25770-1-AP

Synonyme

D0S117E, DBN1, Drebrin, drebrin 1

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Galerie der Validierungsdaten

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  WB analysis using 25770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25770-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 25770-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25770-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 25770-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 25770-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 25770-1-AP (Drebrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 25770-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Drebrin antibody (25770-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 25770-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 25770-1-AP (Drebrin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, Neuro-2a-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen, HepG2-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

25770-1-AP bindet in WB, IF, ELISA Drebrin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Drebrin fusion protein Ag22634
• Vollständiger Name: drebrin 1
• Beobachtetes Molekulargewicht: 100-130 kDa
• GenBank-Zugangsnummer: BC007567
• Gene symbol: DBN1
• Gene ID (NCBI): 1627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

Publikationen

Species	Application	Title
human	IF	Int J Mol Sci; Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery; Authors - Paloma Alvarez-Suarez