Drebrin Monoklonaler Antikörper

Drebrin Monoklonal Antikörper für IF/ICC, IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, IF-P, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1A12H1

Kat-Nr. : CL594-67589

Synonyme

DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 1A12H1

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  IF Staining of mouse brain using CL594-67589

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Drebrin antibody (CL594-67589, Clone: 1A12H1 ) at dilution of 1:200.

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  IF Staining of SH-SY5Y using CL594-67589

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®594 Drebrin antibody (CL594-67589, Clone: 1A12H1 ) at dilution of 1:200.

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  FC experiment of SH-SY5Y using CL594-67589

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Drebrin (CL594-67589, Clone:1A12H1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67589 bindet in IF/ICC, IF-P, FC (Intra) Drebrin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Drebrin fusion protein Ag22635
• Vollständiger Name: drebrin 1
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC007567
• Gene symbol: Drebrin
• Gene ID (NCBI): 1627
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.