Dystroglycan Polyklonaler Antikörper

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• KD/KO Validated

Dystroglycan Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11017-1-PBS

Synonyme

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  WB analysis using 11017-1-AP (same clone as 11017-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11017-1-AP (Dystroglycan antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 11017-1-AP (same clone as 11017-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11017-1-AP (Dystroglycan antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 11017-1-AP (same clone as 11017-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11017-1-AP (Dystroglycan antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11017-1-AP (same clone as 11017-1-PBS)

  IP result of anti-Dystroglycan (IP:11017-1-AP, 4ug; Detection:11017-1-AP 1:500) with mouse brain tissue lysate 2640ug. This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 11017-1-AP (same clone as 11017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 11017-1-AP (same clone as 11017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11017-1-AP (same clone as 11017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11017-1-AP (same clone as 11017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 11017-1-AP (same clone as 11017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 11017-1-AP (same clone as 11017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11017-1-AP (same clone as 11017-1-PBS)

  Immunofluorescent analysis of HeLa cells using 11017-1-AP (Dystroglycan antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 11017-1-AP (same clone as 11017-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Dystroglycan (11017-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11017-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Dystroglycan und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Dystroglycan fusion protein Ag1456
• Vollständiger Name: dystroglycan 1 (dystrophin-associated glycoprotein 1)
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC012740
• Gene symbol: Dystroglycan
• Gene ID (NCBI): 1605
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).